LOCUS       Exported                3904 bp ds-DNA    circular SYN 10-OCT-2014
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    piG0040_regulator_lasR_corrected
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Daniel Gerngross
  TITLE     Direct Submission
  JOURNAL   Exported 10 Oct 2014 from SnapGene 2.5.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     terminator      12..98
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     misc_feature    280..471
                     /note="ROP"
     misc_feature    573..713
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(884..1503)
                     /direction=LEFT
                     /note="pBR322_origin"
     oriT            1523..1631
                     /note="incP origin of transfer"
     CDS             complement(1821..2681)
                     /note="AmpR"
     promoter        complement(2682..2773)
                     /gene="bla"
                     /note="AmpR promoter"
     terminator      2846..2940
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     promoter        2984..3018
                     /note="const. promoter (J23100)"
     RBS             3027..3038
                     /note="RBS (B0034)"
     CDS             3045..3767
                     /codon_start=1
                     /note="lasR (C0179)"
                     /translation="MALVDGFLELERSSGKLEWSAILQKMASDLGFSKILFGLLPKDSQ
                     DYENAFIVGNYPAAWREHYDRAGYARVDPTVSHCTQSVLPIFWEPSIYQTRKQHEFFEE
                     ASAAGLVYGLTMPLHGARGELGALSLSVEAENRAEANRFIESVLPTLWMLKDYALQSGA
                     GLAFEHPVSKPVVLTSREKEVLQWCAIGKTSWEISVICNCSEANVNFHMGNIRRKFGVT
                     SRRVAAIMAVNLGLITL*"
     terminator      3776..3904
                     /note="directional double term (B0015)"
ORIGIN
        1 attaaaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
       61 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgccctag acagctgggc
      121 gcgcccccgc cgcatccata ccgccagttg tttaccctca caacgttcca gtaaccgggc
      181 atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg gtatcattac
      241 ccccatgaac agaaattccc ccttacacgg aggcatcaag tgaccaaaca ggaaaaaacc
      301 gcccttaaca tggcccgctt tatcagaagc cagacattaa cgcttctgga gaaactcaac
      361 gagctggacg cggatgaaca ggcagacatc tgtgaatcgc tgcacgacca cgctgatgag
      421 ctttaccgca gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
      481 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
      541 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat
      601 agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
      661 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
      721 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
      781 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
      841 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
      901 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
      961 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1021 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1081 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1141 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1201 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1261 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1321 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1381 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1441 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1501 tcttttctac ggggccggcc tacggccagc ctcgcagagc aggattcccg ttgagcaccg
     1561 ccaggtgcga ataagggaca gtgaagaagg aacacccgct cgcgggtggg cctacttcac
     1621 ctatcctgcc cggctgacgc cgttggatac accaaggaaa gtctacacga accctttggc
     1681 aaaatcctgt atatcgtgcg aaaaaggatg gatataccga aaaaatcgct ataatgaccc
     1741 cgaagcaggg ttatgcagcg gaaaaggaca agggtcgtcc aaaaaaaaag gctccaaaag
     1801 gagcctttaa ttgtatcggt ttaccaatgt ttaatcaggc tcgcgccaat ttccgcaatc
     1861 tgacggttac gttcatccat cgtcgcctgg ctgccggtgg tataaatcac cacaatacgg
     1921 ctcggtttac catccgggcc cagcgccgca ataatgccac ggctgccacg ttcgcccgca
     1981 ccgcttttat ccgcaataaa ccagccagcc ggcagcgcgc tacgcagcag cggacccgcc
     2041 actttatccg cttccatcca atcaatcagt tgctggcggc ttgccagggt cagcagctca
     2101 cccgtcagca gtttacgcag ggtggtcgcc attgctgccg gcatggtggt atcacgttca
     2161 tcgttcggaa tcgcttcgtt cagttccggt tcccaacgat ccagacgggt cacatgatcg
     2221 cccatgttat gcagaaacgc ggtcagttct ttcggaccgc caatggtcgt cagcagcagg
     2281 ttcgccgcgg tgttatcgct catggtaatc gccgcgctgc acagttcacg cacggtcatg
     2341 ccatcggtca gatgtttttc ggtcaccggg ctatattcca ccagatcgtt ctggctataa
     2401 tgaatacgac ggcccagttg ttcctggccc gcatccacac ggctcagaac cgcaccgcac
     2461 agcagcactt taaaggtgct catcatcgga aaacgttctt ccggacgaaa agattccaga
     2521 attttgccgc tgttcagatc cagttcaata tagcccacgc gcgcacccag ttgatcttcc
     2581 gcatctttca ctttcaccag ggtttccgga tgcgcaaaca ccggcaggca aaacgccgca
     2641 aaaaacggaa tcagcgccac acgaaaatgc tgaatgctca tactcttcct ttttcaatat
     2701 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     2761 aaaaataaac aaatagggga attaaaaaaa agcccgctca ttaggcgggc tactaattta
     2821 aatcgtaatt attggggacc cctggattct caccaataaa aaacgcccgg cggcaaccga
     2881 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc
     2941 caagactagt cgccagggtt ttcccagtca cgacgcggcc gcattgacgg ctagctcagt
     3001 cctaggtaca gtgctagcta ctagagaaag aggagaaata ctagatggcc ttggttgacg
     3061 gttttcttga gctggaacgc tcaagtggaa aattggagtg gagcgccatc ctccagaaga
     3121 tggcgagcga ccttggattc tcgaagatcc tgttcggcct gttgcctaag gacagccagg
     3181 actacgagaa cgccttcatc gtcggcaact acccggccgc ctggcgcgag cattacgacc
     3241 gggctggcta cgcgcgggtc gacccgacgg tcagtcactg tacccagagc gtactgccga
     3301 ttttctggga accgtccatc taccagacgc gaaagcagca cgagttcttc gaggaagcct
     3361 cggccgccgg cctggtgtat gggctgacca tgccgctgca tggtgctcgc ggcgaactcg
     3421 gcgcgctgag cctcagcgtg gaagcggaaa accgggccga ggccaaccgt ttcatagagt
     3481 cggtcctgcc gaccctgtgg atgctcaagg actacgcact gcaaagcggt gccggactgg
     3541 ccttcgaaca tccggtcagc aaaccggtgg ttctgaccag ccgggagaag gaagtgttgc
     3601 agtggtgcgc catcggcaag accagttggg agatatcggt tatctgcaac tgctcggaag
     3661 ccaatgtgaa cttccatatg ggaaatattc ggcggaagtt cggtgtgacc tcccgccgcg
     3721 tagcggccat tatggccgtt aatttgggtc ttattactct ctaataatac tagagccagg
     3781 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     3841 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     3901 tata
//
